Cellwall research in the north of Sweden


Abraham NM, Acar A, Liu L, Gopalakrishnan V, Sutton E, Yarovinsky TO, Jutras BL, Heisig M, Ansari JM, Yadav AK, Jefferson KK, Cava F, Jacobs-Wagner C, Fikrig E

Anaplasma-mediated manipulation of arthropod microbiota to promote infection

Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):E781-E790.

Pubmed Link

Kudrin P, Varik V, Oliveira SR, Beljantseva J, Del Peso Santos T, Dzhygyr I, Rejman D, Cava F, Tenson T, Hauryliuk V.

Subinhibitory Concentrations of Bacteriostatic Antibiotics Induce relA-Dependent and relA-Independent Tolerance to β-Lactams.

Antimicrob Agents Chemother. 2017 Mar 24;61(4).

Pubmed Link

Vanderlinde EM, Strozen TG, Hernández SB, Cava F, Howard SP.

Alterations in Peptidoglycan Cross-Linking Suppress the Secretin Assembly Defect Caused by Mutation of GspA in the Type II Secretion System.

J Bacteriol. 2017 Mar 28;199(8). pii: e00617-16.

Pubmed Link

Terceti MS, Rivas AJ, Alvarez L, Noia M, Cava F, Osorio CR.

rstB Regulates Expression of the Photobacterium damselae subsp. damselae Major Virulence Factors Damselysin, Phobalysin P and Phobalysin C.

Front Microbiol. 2017 Apr 10;8:582.

Pubmed Link

Kumar K, Cava F.

Constraint randomised non-negative factor analysis (CRNNFA): an alternate chemometrics approach for analysing the biochemical data sets.

Analyst. 2017 May 30;142(11):1916-1928.

Pubmed Link

van Teeseling MCF, de Pedro MA, Cava F.

Determinants of Bacterial Morphology: From Fundamentals to Possibilities for Antimicrobial Targeting.

Front Microbiol. 2017 Jul 10;8:1264.

Pubmed Link

Howell M, Aliashkevich A, Salisbury AK, Cava F, Bowman GR, Brown PJB.

Absence of the Polar Organizing Protein PopZ Results in Reduced and Asymmetric Cell Division in Agrobacterium tumefaciens.

J Bacteriol. 2017 Aug 8;199(17).

Pubmed Link

Laura Alvarez, Alena Aliashkevich, Miguel A de Pedro and Felipe Cava

Bacterial secretion of D-arginine controls environmental microbial biodiversity

ISME J 2017

Pubmed Link

Barbara Maciejewska, Karol Źrubek, Akbar Espaillat, Krzysztof P. Rembacz, Magdalena Wiśniewska, Felipe Cava, Grzegorz Dubin, and Zuzanna Drulis- Kawa

Modular endolysin of Burkholderia AP3 phage has the largest lysozyme-like catalytic subunit discovered to date and no catalytic aspartate residue

Scientific Reports 2017

Pubmed Link

Kumar K, Espaillat A and Cava F

PG-Metrics: A chemometric-based approach for classifying bacterial peptidoglycan data sets and uncovering their subjacent chemical variability

Plos One 2017

Pubmed Link

Hernández SB, Cava F.

Environmental roles of microbial amino acid racemases.

Environ Microbiol. 2016 Jun;18(6):1673-85. doi: 10.1111/1462-2920.13072.

Pubmed Link

Espaillat A, Forsmo O, El Biari K, Björk R, Lemaitre B, Trygg J, Cañada FJ, de Pedro MA, Cava F.

Chemometric Analysis of Bacterial Peptidoglycan Reveals Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity.

J Am Chem Soc. 2016 Jul 27;138(29):9193-204. doi: 10.1021/jacs.6b04430. Epub 2016 Jul 13.

Pubmed Link

Ghssein G, Brutesco C, Ouerdane L, Fojcik C, Izaute A, Wang S, Hajjar C, Lobinski R, Lemaire D, Richaud P, Voulhoux R, Espaillat A, Cava F, Pignol D, Borezée-Durant E, Arnoux P.

Biosynthesis of a broad-spectrum nicotianamine-like metallophore in Staphylococcus aureus.

Science. 2016 May 27;352(6289):1105-9. doi: 10.1126/science.aaf1018.

Pubmed Link

Dörr T, Alvarez L, Delgado F, Davis BM, Cava F, Waldor MK.

A cell wall damage response mediated by a sensor kinase/response regulator pair enables beta-lactam tolerance

Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):404-9. doi: 10.1073/pnas.1520333113.

Pubmed Link

Alvarez L, Hernandez SB, de Pedro MA, Cava F.

Ultra-Sensitive, High-Resolution Liquid Chromatography Methods for the High-Throughput Quantitative Analysis of Bacterial Cell Wall Chemistry and Structure.

Methods Mol Biol. 2016;1440:11-27. doi: 10.1007/978-1-4939-3676-2_2.

Pubmed Link

Cuenca M, Pfister SP, Buschor S, Bayramova F, Hernandez SB, Cava F, Kuru E, Van Nieuwenhze MS, Brun YV, Coelho FM, Hapfelmeier S.

D-Alanine-Controlled Transient Intestinal Mono-Colonization with Non-Laboratory-Adapted Commensal E. coli Strain HS.

PLoS One. 2016 Mar 22;11(3):e0151872. doi: 10.1371/journal.pone.0151872. eCollection 2016.

Pubmed Link

Sebastian Felgner1,*, Michael Frahm1, Dino Kocijancic, Manfred Rohde, Denitsa Eckweiler, Agata Bielecka, Emilio Bueno, Felipe Cava, Wolf-Rainer Abraham, Roy Curtiss, Susanne Häussler, Marc Erhardt and Siegfried Weiss

AroA deficient Salmonella Typhimurium – more than an auxotrophic mutant

MBio. 2016 Sep 6;7(5). pii: e01220-16. doi: 10.1128/mBio.01220-16.

Pubmed Link

Maciejewska B, Roszniowski B, Espaillat A, Kęsik-Szeloch A, Majkowska-Skrobek G, Kropinski AM, Briers Y, Cava F, Lavigne R, Drulis-Kawa Z.

Klebsiella phages representing a novel clade of viruses with an unknown DNA modification and biotechnologically interesting enzymes.

Appl Microbiol Biotechnol. 2017 Jan;101(2):673-684

Pubmed Link

Moreno-Guzmán M; García-Carmona L; Molinero-Fernández A; Cava F; López-Gil MA; and Escarpa Miguel A

Bi-enzymatic biosensor for on-site, fast and reliable electrochemical detection of relevant d-amino acids in bacterial samples

Sensor & Actuators B (Chemical) 2016

Pubmed Link

Hernández SB, Cava F, Pucciarelli MG, García-Del Portillo F, de Pedro MA, Casadesús J.

Bile-induced peptidoglycan remodeling in Salmonella enterica.

Environ Microbiol. 2015 Apr;17(4):1081-9. doi: 10.1111/1462-2920.12491. Epub 2014 Jun 24.

Pubmed Link

Möll A, Dörr T, Alvarez L, Davis BM, Cava F, Waldor MK.

A D, D-carboxypeptidase is required for Vibrio cholerae halotolerance.

Environ Microbiol. 2015 Feb;17(2):527-40. doi: 10.1111/1462-2920.12779.

Pubmed Link

Siegrist MS, Aditham AK, Espaillat A, Cameron TA, Whiteside SA, Cava F, Portnoy DA, Bertozzi CR.

Host actin polymerization tunes the cell division cycle of an intracellular pathogen.

Cell Rep. 2015 Apr 28;11(4):499-507. doi: 10.1016/j.celrep.2015.03.046. Epub 2015 Apr 16.

Pubmed Link

van Teeseling MC, Mesman RJ, Kuru E, Espaillat A, Cava F, Brun YV, VanNieuwenhze MS, Kartal B, van Niftrik L.

Anammox Planctomycetes have a peptidoglycan cell wall.

Nat Commun. 2015 May 12;6:6878. doi: 10.1038/ncomms7878.

Pubmed Link

de Pedro MA, Cava F.

Structural constraints and dynamics of bacterial cell wall architecture.

Front Microbiol. 2015 May 8;6:449. doi: 10.3389/fmicb.2015.00449. eCollection 2015. Review.

Pubmed Link

Le Roux F, Wegner KM, Baker-Austin C, Vezzulli L, Osorio CR, Amaro C, Ritchie JM, Defoirdt T, Destoumieux-Garzón D, Blokesch M, Mazel D, Jacq A, Cava F, Gram L, Wendling CC, Strauch E, Kirschner A, Huehn S.

The emergence of Vibrio pathogens in Europe: ecology, evolution, and pathogenesis (Paris, 11-12th March 2015).

Front Microbiol. 2015 Aug 13;6:830. doi: 10.3389/fmicb.2015.00830. eCollection 2015.

Pubmed Link

Desmarais SM, Tropini C, Miguel A, Cava F, Monds RD, de Pedro MA, Huang KC.

High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography.

J Biol Chem. 2015 Oct 14. pii: jbc.M115.661660. [Epub ahead of print]

Pubmed Link

Bernardo-García N, Sánchez-Murcia P, Gago F, Cava F and Hermoso JA.

Structural bioinformatics in broad-spectrum racemases: a new path in antimicrobial research.

Current Organic Chemistry. 19(999):1-1 · August 2015

Pubmed Link

Desmarais SM, Cava F, De Pedro MA, Huang KC (2014)

Isolation and preparation of bacterial cell walls for compositional analysis by ultra performance liquid chromatography

J Vis Exp. 2014 Jan 15;(83). doi: 10.3791/51183.

Pubmed Link

Cava F#, de Pedro MA. (2014)

Peptidoglycan plasticity in bacteria: emerging variability of the murein sacculus and their associated biological functions

Curr Opin Microbiol. 2014 Apr;18C:46-53.

Pubmed Link

Espaillat A, Carrasco-Lopez C, Bernardo-Garcia N, Pietrosemolli N, Otero L H, Alvarez L, de Pedro M A, Pazos F, Davis B M, Waldor M K, Hermoso J A# and Cava F#. (2014)

Structural basis for the broad specificity of a new family of amino acid racemases.

Acta Crystallogr D Biol Crystallogr 2014, 70:79-90

Pubmed Link

Dörr T, Möll A, Chao MC, Cava F, Lam H, Davis BM, Waldor MK. (2014)

Differential requirement for PBP1a and PBP1b in the in vivo and in vitro fitness of Vibrio cholerae.

Infect Immun. 2014 Mar 10.[Epub ahead of print]

Pubmed Link

Alvarez L, Espaillat A, Hermoso J A, de Pedro M A, and Cava F.

Peptidoglycan Remodeling by the Coordinated Action of Multispecific Enzymes

Microb Drug Resist. 2014 May 5.

Pubmed Link

Dörr T, Lam H, Alvarez L, Cava F, Davis BM, Waldor MK.

A Novel Peptidoglycan Binding Protein Crucial for PBP1A-Mediated Cell Wall Biogenesis in Vibrio cholerae.

PLoS Genet. 2014 Jun 19;10(6):e1004433.

Pubmed Link

Moll A, Dörr T, Alvarez L, Chao MC, Davis BM, Cava F, Waldor MK.

Cell separation in Vibrio cholerae is mediated by a single amidase whose action is modulated by two non-redundant activators.

J Bacteriol. 2014 Sep 2

Pubmed Link

Desmarais SM, De Pedro MA, Cava F#, Huang KC# (2013)

Peptidoglycan at its peaks: how chromatographic analyses can reveal bacterial cell wall structure and assembly

Mol Microbiol 89 (1): 1-13.

Pubmed Link

Dorr T*, Cava F*#, Lam H, Davis BM, Waldor MK# (2013)

Substrate specificity of an elongation-specific peptidoglycan endopeptidase and its implications for cell wall architecture and growth of Vibrio cholerae

Mol Microbiol, Jul 9, doi: 10.1111/mmi.12323. [Epub ahead of print].

Pubmed Link

Siegrist MS, Whiteside S, Jewett JC, Aditham A, Cava F, Bertozzi CR (2013)

(D)-amino acid chemical reporters reveal peptidoglycan dynamics of an intracellular pathogen

ACS Chem Biol 8 (3): 500-505.

Pubmed Link

Cava F#, Kuru E, Brun YV, de Pedro MA.

Modes of cell wall growth differentiation in rod-shaped bacteria

Curr Opin Microbiol. 2013 Oct 1

Pubmed Link

Alvarez L, Bricio C, Chahlafi Z, Cava F, Hidalgo A and Berenguer J (2012)

Book Chapter, Regulación y transferencia horizontal de la desnitrificación en

Thermus sp. Avances en el metabolismo del nitrógeno. Evolución e integración de las rutas del metabolismo nitrogenado en el equilibrio biogeoquimico. (ISBN: 978-84-15105-52-7).

Pubmed Link

Ayala JA, Cava F, de Pedro MA. (2012)

Cell Wall Stress-sensing Regulatory Systems in Gram-negative Bacteria.

Stress Response in Microbiology. (ISBN: 978-1-908230-04-1).

Pubmed Link

Horcajo P, de Pedro MA, Cava F (2012)

Peptidoglycan plasticity in bacteria: stress-induced peptidoglycan editing by noncanonical D-amino acids

Microb Drug Resist 18 (3): 306-313.

Pubmed Link

Kuru E, Hughes HV, Brown PJ, Hall E, Tekkam S, Cava F, de Pedro MA, Brun YV, VanNieuwenhze MS (2012)

In situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids

Angew Chem Int Ed Engl 51 (50): 12519-12523.

Pubmed Link

Cava F, de Pedro MA, Lam H, Davis BM, Waldor MK (2011)

Distinct pathways for modification of the bacterial cell wall by non-canonical D-amino acids

EMBO J 30 (16): 3442-3453.

Pubmed Link

Cava F, Lam H, de Pedro MA, Waldor MK (2011)

Emerging knowledge of regulatory roles of D-amino acids in bacteria

Cell Mol Life Sci 68 (5): 817-831.

Pubmed Link

Cava F, Chahlafi Z, Alvarez L and Berenguer J (2009).

Book Chapter

Avances en el metabolismo del nitrógeno. De la genómica y la proteómica a las aplicaciones agronómicas, industriales y medioambientales. 2009 (ISBN: 978-84-8454-806-5).

Pubmed Link

Bolivar JM, Rocha-Martin J, Mateo C, Cava F, Berenguer J, Fernandez-Lafuente R, Guisan JM (2009)

Coating of soluble and immobilized enzymes with ionic polymers: full stabilization of the quaternary structure of multimeric enzymes

Biomacromolecules 10 (4): 742-747.

Pubmed Link

Cava F, Hidalgo A, Berenguer J (2009)

Thermus thermophilus as biological model

Extremophiles 13 (2): 213-231.

Pubmed Link

Lam H, Oh DC, Cava F, Takacs CN, Clardy J, de Pedro MA, Waldor MK (2009)

D-amino acids govern stationary phase cell wall remodeling in bacteria

Science 325 (5947): 1552-1555.

Pubmed Link

Bolivar JM, Mateo C, Rocha-Martin J, Cava F, Berenguer J, Fernandez-Lafuente R and Guisan JM (2009)

The adsorption of multimeric enzymes on very lowly activated supports involves more enzyme subunits: Stabilization of a glutamate dehydrogenase from Thermus thermophilus by immobilization on heterofunctional supports.

Enzyme and Microbial Technology. 44(3):139-144.

Pubmed Link

Bolivar J M, Rocha-Martin J, Mateo C, Cava F, Berenguer J, Vega D, Fernandez-Lafuente R, Guisan J M (2009)

Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization.

Journal of Molecular Catalysis. B, Enzymatic vol. 58 issue 1-4 June, p. 158-163.

Pubmed Link

Bolivar JM, Cava F, Mateo C, Rocha-Martin J, Guisan JM, Berenguer J, Fernandez-Lafuente R (2008)

Immobilization-stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus

Appl Microbiol Biotechnol 80 (1): 49-58.

Pubmed Link

Cava F, de Pedro MA, Blas-Galindo E, Waldo GS, Westblade LF, Berenguer J (2008)

Expression and use of superfolder green fluorescent protein at high temperatures in vivo: a tool to study extreme thermophile biology

Environ Microbiol 10 (3): 605-613.

Pubmed Link

Cava F, Zafra O, da Costa MS, Berenguer J (2008)

The role of the nitrate respiration element of Thermus thermophilus in the control and activity of the denitrification apparatus

Environ Microbiol 10 (2): 522-533.

Pubmed Link

Cava F, Zafra O, Berenguer J (2008)

A cytochrome c containing nitrate reductase plays a role in electron transport for denitrification in Thermus thermophilus without involvement of the bc respiratory complex.

ol Microbiol. 2008 Oct;70(2):507-18.

Pubmed Link

Blas-Galindo E, Cava F, Lopez-Vinas E, Mendieta J, Berenguer J (2007)

Use of a dominant rpsL allele conferring streptomycin dependence for positive and negative selection in Thermus thermophilus

Appl Environ Microbiol 73 (16): 5138-5145.

Pubmed Link

Cava F, Laptenko O, Borukhov S, Chahlafi Z, Blas-Galindo E, Gomez-Puertas P, Berenguer J (2007)

Control of the respiratory metabolism of Thermus thermophilus by the nitrate respiration conjugative element NCE

Mol Microbiol 64 (3): 630-646.

Pubmed Link

Chautard H, Blas-Galindo E, Menguy T, Grand'Moursel L, Cava F, Berenguer J, Delcourt M (2007)

An activity-independent selection system of thermostable protein variants

Nat Methods 4 (11): 919-921.

Pubmed Link

Zafra O, Cava F, Blasco F, Magalon A, Berenguer J (2005)

Membrane-associated maturation of the heterotetrameric nitrate reductase of Thermus thermophilus

J Bacteriol 187 (12): 3990-3996.

Pubmed Link

Cava F, de Pedro MA, Schwarz H, Henne A, Berenguer J (2004)

Binding to pyruvylated compounds as an ancestral mechanism to anchor the outer envelope in primitive bacteria

Mol Microbiol 52 (3): 677-690.

Pubmed Link

Cava F, Zafra O, Magalon A, Blasco F, Berenguer J (2004)

A new type of NADH dehydrogenase specific for nitrate respiration in the extreme thermophile Thermus thermophilus

J Biol Chem 279 (44): 45369-45378.

Pubmed Link

Hidalgo A, Betancor L, Moreno R, Zafra O, Cava F, Fernandez-Lafuente R, Guisan JM, Berenguer J (2004)

Thermus thermophilus as a cell factory for the production of a thermophilic Mn-dependent catalase which fails to be synthesized in an active form in Escherichia coli

Appl Environ Microbiol 70 (7): 3839-3844.

Pubmed Link

Moreno R, Hidalgo A, Cava F, Fernandez-Lafuente R, Guisan JM, Berenguer J (2004)

Use of an antisense RNA strategy to investigate the functional significance of Mn-catalase in the extreme thermophile Thermus thermophilus

J Bacteriol 186 (22): 7804-7806.

Pubmed Link

Perales C, Cava F, Meijer WJ, Berenguer J (2003)

Enhancement of DNA, cDNA synthesis and fidelity at high temperatures by a dimeric single-stranded DNA-binding protein

Nucleic Acids Res 31 (22): 6473-6480.

Pubmed Link

Moreno R, Zafra O, Cava F, Berenguer J (2003)

Development of a gene expression vector for Thermus thermophilus based on the promoter of the respiratory nitrate reductase

Plasmid 49 (1): 2-8.

Pubmed Link

Zafra O, Ramirez S, Castan P, Moreno R, Cava F, Valles C, Caro E, Berenguer J (2002)

A cytochrome c encoded by the nar operon is required for the synthesis of active respiratory nitrate reductase in Thermus thermophilus

FEBS Lett 523 (1-3): 99-102.

Pubmed Link

Castan P, Zafra O, Moreno R, de Pedro MA, Valles C, Cava F, Caro E, Schwarz H, Berenguer J (2002)

The periplasmic space in Thermus thermophilus: evidence from a regulation-defective S-layer mutant overexpressing an alkaline phosphatase

Extremophiles 6 (3): 225-232.

Pubmed Link