CavaLab
Cellwall research in the north of Sweden
CavaLab
Cellwall research in the north of Sweden
Abraham NM, Acar A, Liu L, Gopalakrishnan V, Sutton E, Yarovinsky TO, Jutras BL, Heisig M, Ansari JM, Yadav AK, Jefferson KK, Cava F, Jacobs-Wagner C, Fikrig E
Anaplasma-mediated manipulation of arthropod microbiota to promote infection
Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):E781-E790.
Pubmed LinkKudrin P, Varik V, Oliveira SR, Beljantseva J, Del Peso Santos T, Dzhygyr I, Rejman D, Cava F, Tenson T, Hauryliuk V.
Subinhibitory Concentrations of Bacteriostatic Antibiotics Induce relA-Dependent and relA-Independent Tolerance to β-Lactams.
Antimicrob Agents Chemother. 2017 Mar 24;61(4).
Pubmed LinkVanderlinde EM, Strozen TG, Hernández SB, Cava F, Howard SP.
Alterations in Peptidoglycan Cross-Linking Suppress the Secretin Assembly Defect Caused by Mutation of GspA in the Type II Secretion System.
J Bacteriol. 2017 Mar 28;199(8). pii: e00617-16.
Pubmed LinkTerceti MS, Rivas AJ, Alvarez L, Noia M, Cava F, Osorio CR.
rstB Regulates Expression of the Photobacterium damselae subsp. damselae Major Virulence Factors Damselysin, Phobalysin P and Phobalysin C.
Front Microbiol. 2017 Apr 10;8:582.
Pubmed LinkKumar K, Cava F.
Constraint randomised non-negative factor analysis (CRNNFA): an alternate chemometrics approach for analysing the biochemical data sets.
Analyst. 2017 May 30;142(11):1916-1928.
Pubmed Linkvan Teeseling MCF, de Pedro MA, Cava F.
Determinants of Bacterial Morphology: From Fundamentals to Possibilities for Antimicrobial Targeting.
Front Microbiol. 2017 Jul 10;8:1264.
Pubmed LinkHowell M, Aliashkevich A, Salisbury AK, Cava F, Bowman GR, Brown PJB.
Absence of the Polar Organizing Protein PopZ Results in Reduced and Asymmetric Cell Division in Agrobacterium tumefaciens.
J Bacteriol. 2017 Aug 8;199(17).
Pubmed LinkLaura Alvarez, Alena Aliashkevich, Miguel A de Pedro and Felipe Cava
Bacterial secretion of D-arginine controls environmental microbial biodiversity
ISME J 2017
Pubmed LinkBarbara Maciejewska, Karol Źrubek, Akbar Espaillat, Krzysztof P. Rembacz, Magdalena Wiśniewska, Felipe Cava, Grzegorz Dubin, and Zuzanna Drulis- Kawa
Modular endolysin of Burkholderia AP3 phage has the largest lysozyme-like catalytic subunit discovered to date and no catalytic aspartate residue
Scientific Reports 2017
Pubmed LinkKumar K, Espaillat A and Cava F
PG-Metrics: A chemometric-based approach for classifying bacterial peptidoglycan data sets and uncovering their subjacent chemical variability
Plos One 2017
Pubmed LinkHernández SB, Cava F.
Environmental roles of microbial amino acid racemases.
Environ Microbiol. 2016 Jun;18(6):1673-85. doi: 10.1111/1462-2920.13072.
Pubmed LinkEspaillat A, Forsmo O, El Biari K, Björk R, Lemaitre B, Trygg J, Cañada FJ, de Pedro MA, Cava F.
Chemometric Analysis of Bacterial Peptidoglycan Reveals Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity.
J Am Chem Soc. 2016 Jul 27;138(29):9193-204. doi: 10.1021/jacs.6b04430. Epub 2016 Jul 13.
Pubmed LinkGhssein G, Brutesco C, Ouerdane L, Fojcik C, Izaute A, Wang S, Hajjar C, Lobinski R, Lemaire D, Richaud P, Voulhoux R, Espaillat A, Cava F, Pignol D, Borezée-Durant E, Arnoux P.
Biosynthesis of a broad-spectrum nicotianamine-like metallophore in Staphylococcus aureus.
Science. 2016 May 27;352(6289):1105-9. doi: 10.1126/science.aaf1018.
Pubmed LinkDörr T, Alvarez L, Delgado F, Davis BM, Cava F, Waldor MK.
A cell wall damage response mediated by a sensor kinase/response regulator pair enables beta-lactam tolerance
Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):404-9. doi: 10.1073/pnas.1520333113.
Pubmed LinkAlvarez L, Hernandez SB, de Pedro MA, Cava F.
Ultra-Sensitive, High-Resolution Liquid Chromatography Methods for the High-Throughput Quantitative Analysis of Bacterial Cell Wall Chemistry and Structure.
Methods Mol Biol. 2016;1440:11-27. doi: 10.1007/978-1-4939-3676-2_2.
Pubmed LinkCuenca M, Pfister SP, Buschor S, Bayramova F, Hernandez SB, Cava F, Kuru E, Van Nieuwenhze MS, Brun YV, Coelho FM, Hapfelmeier S.
D-Alanine-Controlled Transient Intestinal Mono-Colonization with Non-Laboratory-Adapted Commensal E. coli Strain HS.
PLoS One. 2016 Mar 22;11(3):e0151872. doi: 10.1371/journal.pone.0151872. eCollection 2016.
Pubmed LinkSebastian Felgner1,*, Michael Frahm1, Dino Kocijancic, Manfred Rohde, Denitsa Eckweiler, Agata Bielecka, Emilio Bueno, Felipe Cava, Wolf-Rainer Abraham, Roy Curtiss, Susanne Häussler, Marc Erhardt and Siegfried Weiss
AroA deficient Salmonella Typhimurium – more than an auxotrophic mutant
MBio. 2016 Sep 6;7(5). pii: e01220-16. doi: 10.1128/mBio.01220-16.
Pubmed LinkMaciejewska B, Roszniowski B, Espaillat A, Kęsik-Szeloch A, Majkowska-Skrobek G, Kropinski AM, Briers Y, Cava F, Lavigne R, Drulis-Kawa Z.
Klebsiella phages representing a novel clade of viruses with an unknown DNA modification and biotechnologically interesting enzymes.
Appl Microbiol Biotechnol. 2017 Jan;101(2):673-684
Pubmed LinkMoreno-Guzmán M; García-Carmona L; Molinero-Fernández A; Cava F; López-Gil MA; and Escarpa Miguel A
Bi-enzymatic biosensor for on-site, fast and reliable electrochemical detection of relevant d-amino acids in bacterial samples
Sensor & Actuators B (Chemical) 2016
Pubmed LinkHernández SB, Cava F, Pucciarelli MG, García-Del Portillo F, de Pedro MA, Casadesús J.
Bile-induced peptidoglycan remodeling in Salmonella enterica.
Environ Microbiol. 2015 Apr;17(4):1081-9. doi: 10.1111/1462-2920.12491. Epub 2014 Jun 24.
Pubmed LinkMöll A, Dörr T, Alvarez L, Davis BM, Cava F, Waldor MK.
A D, D-carboxypeptidase is required for Vibrio cholerae halotolerance.
Environ Microbiol. 2015 Feb;17(2):527-40. doi: 10.1111/1462-2920.12779.
Pubmed LinkSiegrist MS, Aditham AK, Espaillat A, Cameron TA, Whiteside SA, Cava F, Portnoy DA, Bertozzi CR.
Host actin polymerization tunes the cell division cycle of an intracellular pathogen.
Cell Rep. 2015 Apr 28;11(4):499-507. doi: 10.1016/j.celrep.2015.03.046. Epub 2015 Apr 16.
Pubmed Linkvan Teeseling MC, Mesman RJ, Kuru E, Espaillat A, Cava F, Brun YV, VanNieuwenhze MS, Kartal B, van Niftrik L.
Anammox Planctomycetes have a peptidoglycan cell wall.
Nat Commun. 2015 May 12;6:6878. doi: 10.1038/ncomms7878.
Pubmed Linkde Pedro MA, Cava F.
Structural constraints and dynamics of bacterial cell wall architecture.
Front Microbiol. 2015 May 8;6:449. doi: 10.3389/fmicb.2015.00449. eCollection 2015. Review.
Pubmed LinkLe Roux F, Wegner KM, Baker-Austin C, Vezzulli L, Osorio CR, Amaro C, Ritchie JM, Defoirdt T, Destoumieux-Garzón D, Blokesch M, Mazel D, Jacq A, Cava F, Gram L, Wendling CC, Strauch E, Kirschner A, Huehn S.
The emergence of Vibrio pathogens in Europe: ecology, evolution, and pathogenesis (Paris, 11-12th March 2015).
Front Microbiol. 2015 Aug 13;6:830. doi: 10.3389/fmicb.2015.00830. eCollection 2015.
Pubmed LinkDesmarais SM, Tropini C, Miguel A, Cava F, Monds RD, de Pedro MA, Huang KC.
High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography.
J Biol Chem. 2015 Oct 14. pii: jbc.M115.661660. [Epub ahead of print]
Pubmed LinkBernardo-García N, Sánchez-Murcia P, Gago F, Cava F and Hermoso JA.
Structural bioinformatics in broad-spectrum racemases: a new path in antimicrobial research.
Current Organic Chemistry. 19(999):1-1 · August 2015
Pubmed LinkDesmarais SM, Cava F, De Pedro MA, Huang KC (2014)
Isolation and preparation of bacterial cell walls for compositional analysis by ultra performance liquid chromatography
J Vis Exp. 2014 Jan 15;(83). doi: 10.3791/51183.
Pubmed LinkCava F#, de Pedro MA. (2014)
Peptidoglycan plasticity in bacteria: emerging variability of the murein sacculus and their associated biological functions
Curr Opin Microbiol. 2014 Apr;18C:46-53.
Pubmed LinkEspaillat A, Carrasco-Lopez C, Bernardo-Garcia N, Pietrosemolli N, Otero L H, Alvarez L, de Pedro M A, Pazos F, Davis B M, Waldor M K, Hermoso J A# and Cava F#. (2014)
Structural basis for the broad specificity of a new family of amino acid racemases.
Acta Crystallogr D Biol Crystallogr 2014, 70:79-90
Pubmed LinkDörr T, Möll A, Chao MC, Cava F, Lam H, Davis BM, Waldor MK. (2014)
Differential requirement for PBP1a and PBP1b in the in vivo and in vitro fitness of Vibrio cholerae.
Infect Immun. 2014 Mar 10.[Epub ahead of print]
Pubmed LinkAlvarez L, Espaillat A, Hermoso J A, de Pedro M A, and Cava F.
Peptidoglycan Remodeling by the Coordinated Action of Multispecific Enzymes
Microb Drug Resist. 2014 May 5.
Pubmed LinkDörr T, Lam H, Alvarez L, Cava F, Davis BM, Waldor MK.
A Novel Peptidoglycan Binding Protein Crucial for PBP1A-Mediated Cell Wall Biogenesis in Vibrio cholerae.
PLoS Genet. 2014 Jun 19;10(6):e1004433.
Pubmed LinkMoll A, Dörr T, Alvarez L, Chao MC, Davis BM, Cava F, Waldor MK.
Cell separation in Vibrio cholerae is mediated by a single amidase whose action is modulated by two non-redundant activators.
J Bacteriol. 2014 Sep 2
Pubmed LinkDesmarais SM, De Pedro MA, Cava F#, Huang KC# (2013)
Peptidoglycan at its peaks: how chromatographic analyses can reveal bacterial cell wall structure and assembly
Mol Microbiol 89 (1): 1-13.
Pubmed LinkDorr T*, Cava F*#, Lam H, Davis BM, Waldor MK# (2013)
Substrate specificity of an elongation-specific peptidoglycan endopeptidase and its implications for cell wall architecture and growth of Vibrio cholerae
Mol Microbiol, Jul 9, doi: 10.1111/mmi.12323. [Epub ahead of print].
Pubmed LinkSiegrist MS, Whiteside S, Jewett JC, Aditham A, Cava F, Bertozzi CR (2013)
(D)-amino acid chemical reporters reveal peptidoglycan dynamics of an intracellular pathogen
ACS Chem Biol 8 (3): 500-505.
Pubmed LinkCava F#, Kuru E, Brun YV, de Pedro MA.
Modes of cell wall growth differentiation in rod-shaped bacteria
Curr Opin Microbiol. 2013 Oct 1
Pubmed LinkAlvarez L, Bricio C, Chahlafi Z, Cava F, Hidalgo A and Berenguer J (2012)
Book Chapter, Regulación y transferencia horizontal de la desnitrificación en
Thermus sp. Avances en el metabolismo del nitrógeno. Evolución e integración de las rutas del metabolismo nitrogenado en el equilibrio biogeoquimico. (ISBN: 978-84-15105-52-7).
Pubmed LinkAyala JA, Cava F, de Pedro MA. (2012)
Cell Wall Stress-sensing Regulatory Systems in Gram-negative Bacteria.
Stress Response in Microbiology. (ISBN: 978-1-908230-04-1).
Pubmed LinkHorcajo P, de Pedro MA, Cava F (2012)
Peptidoglycan plasticity in bacteria: stress-induced peptidoglycan editing by noncanonical D-amino acids
Microb Drug Resist 18 (3): 306-313.
Pubmed LinkKuru E, Hughes HV, Brown PJ, Hall E, Tekkam S, Cava F, de Pedro MA, Brun YV, VanNieuwenhze MS (2012)
In situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids
Angew Chem Int Ed Engl 51 (50): 12519-12523.
Pubmed LinkCava F, de Pedro MA, Lam H, Davis BM, Waldor MK (2011)
Distinct pathways for modification of the bacterial cell wall by non-canonical D-amino acids
EMBO J 30 (16): 3442-3453.
Pubmed LinkCava F, Lam H, de Pedro MA, Waldor MK (2011)
Emerging knowledge of regulatory roles of D-amino acids in bacteria
Cell Mol Life Sci 68 (5): 817-831.
Pubmed LinkCava F, Chahlafi Z, Alvarez L and Berenguer J (2009).
Book Chapter
Avances en el metabolismo del nitrógeno. De la genómica y la proteómica a las aplicaciones agronómicas, industriales y medioambientales. 2009 (ISBN: 978-84-8454-806-5).
Pubmed LinkBolivar JM, Rocha-Martin J, Mateo C, Cava F, Berenguer J, Fernandez-Lafuente R, Guisan JM (2009)
Coating of soluble and immobilized enzymes with ionic polymers: full stabilization of the quaternary structure of multimeric enzymes
Biomacromolecules 10 (4): 742-747.
Pubmed LinkCava F, Hidalgo A, Berenguer J (2009)
Thermus thermophilus as biological model
Extremophiles 13 (2): 213-231.
Pubmed LinkLam H, Oh DC, Cava F, Takacs CN, Clardy J, de Pedro MA, Waldor MK (2009)
D-amino acids govern stationary phase cell wall remodeling in bacteria
Science 325 (5947): 1552-1555.
Pubmed LinkBolivar JM, Mateo C, Rocha-Martin J, Cava F, Berenguer J, Fernandez-Lafuente R and Guisan JM (2009)
The adsorption of multimeric enzymes on very lowly activated supports involves more enzyme subunits: Stabilization of a glutamate dehydrogenase from Thermus thermophilus by immobilization on heterofunctional supports.
Enzyme and Microbial Technology. 44(3):139-144.
Pubmed LinkBolivar J M, Rocha-Martin J, Mateo C, Cava F, Berenguer J, Vega D, Fernandez-Lafuente R, Guisan J M (2009)
Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization.
Journal of Molecular Catalysis. B, Enzymatic vol. 58 issue 1-4 June, p. 158-163.
Pubmed LinkBolivar JM, Cava F, Mateo C, Rocha-Martin J, Guisan JM, Berenguer J, Fernandez-Lafuente R (2008)
Immobilization-stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus
Appl Microbiol Biotechnol 80 (1): 49-58.
Pubmed LinkCava F, de Pedro MA, Blas-Galindo E, Waldo GS, Westblade LF, Berenguer J (2008)
Expression and use of superfolder green fluorescent protein at high temperatures in vivo: a tool to study extreme thermophile biology
Environ Microbiol 10 (3): 605-613.
Pubmed LinkCava F, Zafra O, da Costa MS, Berenguer J (2008)
The role of the nitrate respiration element of Thermus thermophilus in the control and activity of the denitrification apparatus
Environ Microbiol 10 (2): 522-533.
Pubmed LinkCava F, Zafra O, Berenguer J (2008)
A cytochrome c containing nitrate reductase plays a role in electron transport for denitrification in Thermus thermophilus without involvement of the bc respiratory complex.
ol Microbiol. 2008 Oct;70(2):507-18.
Pubmed LinkBlas-Galindo E, Cava F, Lopez-Vinas E, Mendieta J, Berenguer J (2007)
Use of a dominant rpsL allele conferring streptomycin dependence for positive and negative selection in Thermus thermophilus
Appl Environ Microbiol 73 (16): 5138-5145.
Pubmed LinkCava F, Laptenko O, Borukhov S, Chahlafi Z, Blas-Galindo E, Gomez-Puertas P, Berenguer J (2007)
Control of the respiratory metabolism of Thermus thermophilus by the nitrate respiration conjugative element NCE
Mol Microbiol 64 (3): 630-646.
Pubmed LinkChautard H, Blas-Galindo E, Menguy T, Grand'Moursel L, Cava F, Berenguer J, Delcourt M (2007)
An activity-independent selection system of thermostable protein variants
Nat Methods 4 (11): 919-921.
Pubmed LinkZafra O, Cava F, Blasco F, Magalon A, Berenguer J (2005)
Membrane-associated maturation of the heterotetrameric nitrate reductase of Thermus thermophilus
J Bacteriol 187 (12): 3990-3996.
Pubmed LinkCava F, de Pedro MA, Schwarz H, Henne A, Berenguer J (2004)
Binding to pyruvylated compounds as an ancestral mechanism to anchor the outer envelope in primitive bacteria
Mol Microbiol 52 (3): 677-690.
Pubmed LinkCava F, Zafra O, Magalon A, Blasco F, Berenguer J (2004)
A new type of NADH dehydrogenase specific for nitrate respiration in the extreme thermophile Thermus thermophilus
J Biol Chem 279 (44): 45369-45378.
Pubmed LinkHidalgo A, Betancor L, Moreno R, Zafra O, Cava F, Fernandez-Lafuente R, Guisan JM, Berenguer J (2004)
Thermus thermophilus as a cell factory for the production of a thermophilic Mn-dependent catalase which fails to be synthesized in an active form in Escherichia coli
Appl Environ Microbiol 70 (7): 3839-3844.
Pubmed LinkMoreno R, Hidalgo A, Cava F, Fernandez-Lafuente R, Guisan JM, Berenguer J (2004)
Use of an antisense RNA strategy to investigate the functional significance of Mn-catalase in the extreme thermophile Thermus thermophilus
J Bacteriol 186 (22): 7804-7806.
Pubmed LinkPerales C, Cava F, Meijer WJ, Berenguer J (2003)
Enhancement of DNA, cDNA synthesis and fidelity at high temperatures by a dimeric single-stranded DNA-binding protein
Nucleic Acids Res 31 (22): 6473-6480.
Pubmed LinkMoreno R, Zafra O, Cava F, Berenguer J (2003)
Development of a gene expression vector for Thermus thermophilus based on the promoter of the respiratory nitrate reductase
Plasmid 49 (1): 2-8.
Pubmed LinkZafra O, Ramirez S, Castan P, Moreno R, Cava F, Valles C, Caro E, Berenguer J (2002)
A cytochrome c encoded by the nar operon is required for the synthesis of active respiratory nitrate reductase in Thermus thermophilus
FEBS Lett 523 (1-3): 99-102.
Pubmed LinkCastan P, Zafra O, Moreno R, de Pedro MA, Valles C, Cava F, Caro E, Schwarz H, Berenguer J (2002)
The periplasmic space in Thermus thermophilus: evidence from a regulation-defective S-layer mutant overexpressing an alkaline phosphatase
Extremophiles 6 (3): 225-232.
Pubmed Link
